Pleiotropy
Figure 2 shows a funnel plot displaying the MR estimates of individual genetic variants against their precession. Aysmmetry in the funnel plot may arrise due to some genetic variants having unusally strong effects on the outcome, which is indicative of directional pleiotropy.
A Cochrans Q heterogeneity test can be used to formaly assesse for the presence of heterogenity (Table 6).
Table 6: Heterogenity Tests
The intercept of the MR-Regression model captures the average pleitropic affect across all genetic variants (Table 7).
Table 7: MR Egger test for directional pleitropy
Pleiotropy was also assesed using Mendelian Randomization Pleiotropy RESidual Sum and Outlier (MR-PRESSO), that allows for the evlation of evaluation of horizontal pleiotropy in a standared MR model (Table 8). MR-PRESSO performs a global test for detection of horizontal pleiotropy and correction of pleiotropy via outlier removal (Table 9).
Table 8: MR-PRESSO Global Test for pleitropy
Table 9: MR Estimates after MR-PRESSO outlier removal
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